POPLC Optimizer Software for isocratic Methods
Optimize / Develop HPLC Seperations during your Coffee break!
Increasing productivity, confidence in results and costeffectiveness are todays key objectives in HPLC laboratories. POPLC® Optimizer is developed to meet these challenges as a universal software for any type of Liquid Chromatography independent from the brand of instrument or the brand of columns.
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Fig. 1 shows a separation predicted by POPLC® Optimizer using a combination of conventional HPLC columns. None of these columns was able to separate the sample by it`s own. The best possible separation showed co-elution, for some compounds, on each column.
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To improve the separation the listed columns are virtually divided in twenty five 10 mm segments. The POPLC® Optimizer software will calculate each possible segment combination (in this example 736,286 combinations). The result is an improved isocratic separation using the same parameters. Fig.2 shows the best resolution for this sample using the column combination virtually segmented.
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Since only a few column dimensions exist in the market the calculated lenght for the different stationary phases must be adapted to the availability. In our example (Fig.2 ) the 60 mm C18 EPS column is translated into 75 mm. The 160 mm Phenyl is translated into 150 mm and the 10 mm CN into 30 mm. With these dimensions the calculation gives a similar chromatogram (Fig.3) compared to the chromatogram in Fig.2.
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In Fig.4 a combination of column segments is illustrated which is not virtual. They are available in the unique and patented POPLC® Basic-Kits and can be used instead of regular columns. POPLC® Optimizer software will predict the optimized column and selectivity combination. The Kits allow the immidiate proof of prediction. The measured retention times will have a small deviation from the predicted retention times. That means you are able to predict the most selective and fast separation without running an instrument for optimization.
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Download (works in Demo Mode without Dongle)
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LG POPLC Optimizer Software for gradient Methods
To improve the selectivity and to shorten the analysis time by performing gradient instead of isocratic elution in phase optimized liquid chromatography.
Advantages:
- Multiple gradient steps (up to 6) can be defined.
- Definition of the types of segments is flexible, i.e. from 2 to 6.
- Combined optimization restriction on both total column length and analysis time.
- Convenient project management.
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