ProntoSIL 300-5-C18H
 Part number:
2546K185PS050
 Dimension:
250 x 4.6 mm
 Eluent:
A: H2O/0.1% TFA 
B: ACN/ H2O, 95/5 (v/v) 0.1% TFA
 Gradient:
25-80% B, 0-17 min
 Flow:
1.5 ml/min
 Detection:
UV 220 nm
 Temperature:
30 °C
 Injection:
5 µl
 Sample:
1: Protamine Sulfate 
2: Phenol 
3: Cresol 
4: Insulin H 
5: Protamine insulin