HC-40 The Hamilton HC-40 material is a strong cation exchanger based on polystyrene / divinylbenzene. This soft gel column with a degree of cross-linking of 4% is used for the ligand-chromatographic separation of carbohydrates. With these columns, it is possible to separate even oligosaccharides up to DP8 under the simplest separation conditions - as mobile phase only pure water at high temperatures is used. For detection, the RI detector is usually used for these separations. This material is available only in the calcium form (USP L19). The filler material is only available in columns with an inner diameter of 7.8 mm and in stainless steel design.