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SUPER FRACTION COLLECTOR

HPLC / LC and general liquid handling - Stand-Alone - Micro Computer Controlled

From open column to HPLC liquid chromatography and general liquid samplings, the Super Fraction Collector, model SF-3120 has a wide range of uses.The SF-3120 is a fraction collector with increased reliability, employs an X-Y movement mechanism based on long years of technological accumulation, a new vertical movement apparatus and a large display screen for easier use than ever. The SF-3120 holds up to 120 vessels, and can handle a variety of vessel diameters and lengths. With its numerous fraction functions, the SF-3120 boasts a wide range of applications.

FRACTION COLLECTOR SF-3120
FRACTION COLLECTOR SF-3120

Features:

  • Easier operation with a large display screen
With 9 operation keys and a large 40 characters x 8 row display screen, it is easier than ever to input programming and parameter settings. The graph display of chromatography signals makes it easy to determine the status of fraction collection.
  • Reliable construction
The water-sensitive operation panel as well as the mechanical and electronic parts are placed behind the test tube rack to protect against water droplets for a practical, long-lasting design.

  • Supports different sized collection vessels
The vertical dial allows 70mm of movement to adjust the dropper nozzle position to the test tube length (105 ~ 180mm). The standard test tube rack will hold 120 tubes with a diameter of 12 ~15mm (maximum of 18.1mm with
the standard rack holder removed). Large volume vessels can be set when the rack lid is removed.
  • Quick nozzle movement with higher resolution
A sturdy frame and higher resolution stepping motor are used. The nozzle moves accurately to the set position, from tube to tube in 0.1 seconds.

  • Unneeded constituents drained
Waste constituents are drained out so test tubes are not unnecessarily
contaminated.
  • Safe, chemically resistant material
The exterior of the unit is made of flame retardant ABS plastic, and the test tube rack and drain are made of chemically resistant polypropylene.

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Main body and standard rack
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For collecting big bottles

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Drain
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Simple up/down Movement with rotary knob

Loaded with Unique Collection Functions

Simple mode: Collect by time, drops, volume or signal.
Manual mode:
Collect fractions while watching the chromatography signals displayed on the screen. A fraction may be reviewed under playback mode.

Peak Mode: Specify up to a maximum of 10 peaks for identification. Peak determination maybe made using a combination of absolute value and slope angle.
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Example of peak absolute value setting
Set the percent value using an input signal full scale of 100%. Peaks above the set absolute value are identified.
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Example of slope angle setting
As shown in the graph at right, because of baseline drift, only three peaks are identified at 1,2/3 and 4/5/6/7/8 by absolute value setting. However, by using a slope setting, all 8 peaks are identified.

Window mode

A maximum of 16 windows (time ranges) may be set so that peaks are identified and fraction collection occurs only within each window. By setting the monitor
time, non-peaks may also be collected in the test tubes.

Window mode setting, example 1
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Window 1 is set between 2min00sec and 2min32sec at request time of 12sec. Window 2 is set between 3min32sec and 5min00sec at 8 sec request time.
  • Main settings: Wait time: [40 sec]
    Non-window monitor time: [0 sec]
  • Window settings:
    01: Start [2min00sec] ~ End [2min32sec]
    Request time [12 sec]
    02: Start [3min32sec] ~ End [5min00sec]
    Request time [8 sec]
  • Peak parameters:  Absolute value: [0%],
    Slope angle: [0mv/min]
    Response: [*HIGH], Delay time: [0 sec]
Window mode setting, example 2 (peak identification)
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Window mode setting, example 2 (peak identification)
Peaks within the windows are identified. Fraction collection is set at 12 seconds during peaks for window 1. For non-peaks, the monitor time interval is 8 seconds.
  • Main settings: Wait time: [40 sec]
    Non-window monitor time: [0 sec]
  • Window settings:
    01: Start [2min00sec] ~ End [2min32sec]
    Request time [12 sec]
    Monitor [08 sec]
    02: Start [3min32sec] ~ End [5min00sec]
    Request time [8 sec]
    Monitor [24 sec]
  • Peak parameters: Absolute value: [40%]
    Slope angle: [20mV/min]
    Response: [*HIGH], Delay time: [0 sec]

Sampling mode
Collect fractions for a set interval during a set period. Multiple collections may be set per test tube. In the setting example shown on the right, wait time is 5 minutes, each test tube has 2 repeats of 5 minutes intervals every 15 minutes.

Setting Example = Sampling Mode

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Multiple sample functions
After a fraction collection has completed, the next fraction sampling begins at the next test tube or after skipping one tube
Synchronize the chromatogram and collection
using the time delay function

Because of the distance from the UV absorption detector, refractometer or other instruments to the fraction collector dispense nozzle, there is a delay from the point of graphing until the sample
is collected in the tube. By inputting the lag time,
the collected sample can be set to accurately conform to the record (chromatogram).
Verify the fraction collection results by looking at the graph
The results of up to 500 events can be displayed.
Chromatography signals are displayed in peak, window and manual modes.

Fraction result display

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Peak signal graph display

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Operate using a computer
Using a computer , the parameter settings for each mode as well as starting, ending, pausing and advancing can be controlled. By loading up to 500 fraction results into the computer, the operation history of the equipment and test results can be easily verified
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Sample liquid chromatography system set-up
After inserting the sample into the top of the column, the elute separates out. While characteristics are measured with detectors,  the separated substances are eluted into the fraction collectors
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Example of sampling system set-up
A small amount is sampled at a fixed interval, and inserted into the fraction
collectors

SF-3120 Accessories

Optional racks and rack accessories Non-standard test tube racks available (* Microplates, scintillation vials and test tubes are not included with racks)
96 well microplate Rack
96 well microplate
Holding qty: 4
27.5 mm vial Rack
27.5 mm (OD) X 57mm
scintillation vials
Holding qty: 56
16.5 mm vial Rack
16.5 mm (OD) x 54mm
scintillation vials
Holing qty: 120
Test tube Rack (without holder) for 15 mm OD and up test tube
15 – 18.1mm (OD) x 105 – 180mm (L) test tubes
Holding qty: 120
Test tube Rack (with 12mm OD holder) for 12 mm OD test tube
12 mm (OD) x 105 – 180mm (L) test tubes
Holding qty: 120

12 mm holder
Test Tube rack (12mm OD holder), holds 30 12-mm test tubes
Rack middle plate
Place in the test tube rack (standard, 15mm OD or larger, or 12mm OD) to allow use of 35~105 (L) mm tubes

Eppendorf tube adapter Use to adapt the standard test tube rack to Eppendorf tubes
(*Test tube rack and Eppendorf tubes are not included with the Eppendorf tube adapter)
  • Max. no. of fractions: 120
  • Useable test tubes: 1.5 mL (40mm high, 11mm body circumference)
  • Material: Stainless Steel
  • Dimensions: 275 (w) x 248 (d) x 115 (h) mm

Preparative Options
Prep. Funnel  and Prep. Funnel Rack allow the end user to collect fractions in large volume containers.
Prep. Funnel
30 fraction funnels in each Prep.Funnel
Prep. Funnel Rack
Holds up to 3 Prep. Funnels (3x30 pos.)
Prep. Tygon Tubing
TYGON TUBE (6.35X9.52mm) X 5M)
Prep. Mobile Cart
Cut out hole on the Top table

3-Way diverter valve
Prevent the sample from spillage out from the tubes while the nozzle is in transit. Also, use the included seal adapter to use the valve as a stop valve. May be set to drain to prevent residual sample in the valve from the previous peak from being mixed in with the next fraction. The low residual 3-way valve has a low residual volume to decrease contamination. (*The low residual valve has a flow volume approximately 40% less than the diverter valve)
3-Way diverter valve
Inlet pressure: 300kPa (40.5psi)
Connector Material: PTFE, PCTFE
Residual volume (μL):
IN: 26 - NC: 66 - NO: 66
Power consumption: 2.2 VA max
Low residual 3-Way valve
Inlet pressure: 200kPa (29psi)
Connector Material: PTFE, PCTFE
Residual volume (μL):
IN: 14 - NC: 7 - NO: 7
Power consumption: 2.2 VA max

Specifications

Model

SF-3120

Test tube quantity

120 tubes (12 ~ 18.1 mm OD x 105 ~ 180 mm L)(remove tube holders for larger than 15 mm OD)

Operation modes

Simple, Peak, Window, Manual, Sampling

Collection methods

By time, Drop count, Drop volume, Signal

Nozzle shift time

0.1 sec (tube center to center)

Modes of Operation

Simple mode

  • Time: 99min 59 sec /tube max.
  • Drop: 9999 count / tube max.
  • Volume: 999.9 ml / tube max.
  • Signal: 9999 count / tube max.
  • Peak mode:
    End time: 999 min max.
    Request time: 99 min 59 sec / tube max.
    Monitor time: 99 min 59 sec / tube max.
    Fraction peakr: Up to 10 out of 99 peaks
    Peak discrimination: Absolute value 100% max.
    Slope 99.99mV / min or 9.999mV max.
  • Signal response: Chromato signal sampling
    High: 0.5 sec Low: 3 sec

Window mode

  • Non-window monitor: 99 min 59 sec max.
  • No. of windows: 16 max
  • Start / end time: 999 min 59 sec max.
  • Request / Monitor time: 99 min 59 sec / tube max.
  • Peak specifications: Same as in peak mode

Manual mode

  • Learning response: Chromato. Signal display
    High: 0.5 sec Low: 3 sec.
  • Playback: Replay results of learning

Sampling mode

  • Interval: 99 min 59 sec max.
  • Collection time: 99 min 59 sec max.
  • Collection volume: 999.9mL max.
  • No. of repeats: 99 max.

Common parameters

  • Wait time: 999 min 0 sec max.
    for Simple, Peak, Window and Sampling modes

Equipment parameter specifications

Volume collection

  • Collection time (min) =
    Flow volume (mL) / flow rate (mL/min)

Multi-sample

  • Skip one vessel or continuous (Non-skip)

Marker (2)

  • ON after operation (assembled with I/O extension unit, pumps stops after operation) or OFF

Power failure

  • Display On or Off

RS-2323C

  • Baud rates 1200, 2400, 4800
    Characters: 8-bit, Parity: None, Stop: 2-bit

Vessel parameter specifications

Rack selection

  • Standard test tube Rack
  • Eppendorf tube Rack
  • Scintillation vial (16.5mm) Rack
  • Scintillation vial (27.5mm) Rack
  • Microtiter plate (96well x4) Rack
  • Prep. Funnel Rack

Bottle position arrangement

Bottle arrangement

  • Matrix arrangement: 2 patterns
  • Arbitrary arrangement: 1 pattern

Regular arrangement

  • Max. 12 rows x 15 columns (120 pos. max.)

Arbitrary arrangement

  • Max. 100 positions

External signals

Event marker

  • 2 contact point output circuits:
  • 1. Pulse signals
  • 2. Level signals

Chromato, signal input

  • 10 mV or 1 V full scale

External start input

  • ON START

External end input

  • ON END

External count input

  • ON COUNT

Serial interface

  • RS-232C (9-pin connector)

Accessories

  • Test tube rack (1)
  • Drain (1)
  • Dust cover (1)
  • I/O terminal connector (1)
  • Sample tubing:
    PTFE 2 mm OD x 1 mm ID x 1M (1)
  • Drain tubing:
  • Silicon 8mm OD x 5 mm x 0.5 M (1)
  • Tube supsender (2)
  • Power cord (1)
  • Installation & Operation Manual (1)

General electrical specifications

Clock

  • Quartz oscillation

Operation Display

  • 40 characters x 8 rows (LCD w/backlight)

Parameter memory

  • Battery back-up

Temperature range

  • 2 ~ 40°C (35~104°F)

Power

  • AC 100 V, 115 V, 240 V, 0.23 A max.

External dimensions

· 286 (w) x 387 (d) x 335 (h) mm

Weight

· Approx. 7 kg (15.4 lbs)


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