SUPER FRACTION COLLECTOR
HPLC / LC and general liquid handling - Stand-Alone - Micro Computer Controlled
Features:
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the standard rack holder removed). Large volume vessels can be set when the rack lid is removed. |
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contaminated. |
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Loaded with Unique Collection Functions
Simple mode: Collect by time, drops, volume or signal.
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Manual mode:
Collect fractions while watching the chromatography signals displayed on the screen. A fraction may be reviewed under playback mode. |
Example of peak absolute value setting
Set the percent value using an input signal full scale of 100%. Peaks above the set absolute value are identified. |
Example of slope angle setting
As shown in the graph at right, because of baseline drift, only three peaks are identified at 1,2/3 and 4/5/6/7/8 by absolute value setting. However, by using a slope setting, all 8 peaks are identified. |
Window mode
time, non-peaks may also be collected in the test tubes.
Window mode setting, example 1
Window 1 is set between 2min00sec and 2min32sec at request time of 12sec. Window 2 is set between 3min32sec and 5min00sec at 8 sec request time.
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Window mode setting, example 2 (peak identification)
Window mode setting, example 2 (peak identification)
Peaks within the windows are identified. Fraction collection is set at 12 seconds during peaks for window 1. For non-peaks, the monitor time interval is 8 seconds.
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Multiple sample functions
After a fraction collection has completed, the next fraction sampling begins at the next test tube or after skipping one tube |
Synchronize the chromatogram and collection
using the time delay function Because of the distance from the UV absorption detector, refractometer or other instruments to the fraction collector dispense nozzle, there is a delay from the point of graphing until the sample is collected in the tube. By inputting the lag time, the collected sample can be set to accurately conform to the record (chromatogram). |
Verify the fraction collection results by looking at the graph
The results of up to 500 events can be displayed. Chromatography signals are displayed in peak, window and manual modes. |
Sample liquid chromatography system set-up
After inserting the sample into the top of the column, the elute separates out. While characteristics are measured with detectors, the separated substances are eluted into the fraction collectors |
Example of sampling system set-up
A small amount is sampled at a fixed interval, and inserted into the fraction collectors |
SF-3120 Accessories
96 well microplate Rack
96 well microplate Holding qty: 4 |
27.5 mm vial Rack
27.5 mm (OD) X 57mm scintillation vials Holding qty: 56 |
16.5 mm vial Rack
16.5 mm (OD) x 54mm scintillation vials Holing qty: 120 |
Test tube Rack (without holder) for 15 mm OD and up test tube
15 – 18.1mm (OD) x 105 – 180mm (L) test tubes Holding qty: 120 |
Test tube Rack (with 12mm OD holder) for 12 mm OD test tube
12 mm (OD) x 105 – 180mm (L) test tubes Holding qty: 120 |
12 mm holder
Test Tube rack (12mm OD holder), holds 30 12-mm test tubes |
Rack middle plate
Place in the test tube rack (standard, 15mm OD or larger, or 12mm OD) to allow use of 35~105 (L) mm tubes |
(*Test tube rack and Eppendorf tubes are not included with the Eppendorf tube adapter)
- Max. no. of fractions: 120
- Useable test tubes: 1.5 mL (40mm high, 11mm body circumference)
- Material: Stainless Steel
- Dimensions: 275 (w) x 248 (d) x 115 (h) mm
Prep. Funnel and Prep. Funnel Rack allow the end user to collect fractions in large volume containers.
Prep. Funnel
30 fraction funnels in each Prep.Funnel |
Prep. Funnel Rack
Holds up to 3 Prep. Funnels (3x30 pos.) |
Prep. Tygon Tubing
TYGON TUBE (6.35X9.52mm) X 5M) |
Prep. Mobile Cart
Cut out hole on the Top table |
Prevent the sample from spillage out from the tubes while the nozzle is in transit. Also, use the included seal adapter to use the valve as a stop valve. May be set to drain to prevent residual sample in the valve from the previous peak from being mixed in with the next fraction. The low residual 3-way valve has a low residual volume to decrease contamination. (*The low residual valve has a flow volume approximately 40% less than the diverter valve)
3-Way diverter valve
Inlet pressure: 300kPa (40.5psi) Connector Material: PTFE, PCTFE Residual volume (μL): IN: 26 - NC: 66 - NO: 66 Power consumption: 2.2 VA max |
Low residual 3-Way valve
Inlet pressure: 200kPa (29psi) Connector Material: PTFE, PCTFE Residual volume (μL): IN: 14 - NC: 7 - NO: 7 Power consumption: 2.2 VA max |
Specifications
Model |
SF-3120 |
Test tube quantity |
120 tubes (12 ~ 18.1 mm OD x 105 ~ 180 mm L)(remove tube holders for larger than 15 mm OD) |
Operation modes |
Simple, Peak, Window, Manual, Sampling |
Collection methods |
By time, Drop count, Drop volume, Signal |
Nozzle shift time |
0.1 sec (tube center to center) |
Modes of Operation |
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Simple mode |
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Window mode |
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Manual mode |
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Sampling mode |
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Common parameters |
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Equipment parameter specifications |
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Volume collection |
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Multi-sample |
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Marker (2) |
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Power failure |
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RS-2323C |
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Vessel parameter specifications |
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Rack selection |
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Bottle position arrangement |
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Bottle arrangement |
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Regular arrangement |
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Arbitrary arrangement |
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External signals |
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Event marker |
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Chromato, signal input |
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External start input |
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External end input |
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External count input |
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Serial interface |
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Accessories |
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General electrical specifications |
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Clock |
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Operation Display |
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Parameter memory |
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Temperature range |
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Power |
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External dimensions |
· 286 (w) x 387 (d) x 335 (h) mm |
Weight |
· Approx. 7 kg (15.4 lbs) |