Cold Medicine Gradient Separation / Method / Application in HPLC / LC

 COLUMN: ProntoSIL Eurobond C18 5 µl
 Part number:
1204F181PS050
 Dimension:
125 x 4.0 mm
 Eluent:
A: 5 mM Li2SO4 in H2O/H2SO4; pH 2.1 
B: ACN/50 mM H3PO4
 Gradient:
0% B, 0-120 s; 
0-12% B, 120-184 s; 
12-17% B, 184-500 s; 
17-39% B, 500-720 s
 Flow:
1 ml/min
 Detection:
UV 230 nm, 0-240 s;
UV 261 nm, 240-720 s
 Temperature:
20 °C
 Injection:
3 µl
 Sample:
1: Ascorbic acid 
2: Paracetamol 
3: Coffein 
4: Chlorphenamin